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Industrial Liaison Group:
Tel: +44 (0) 1235 778797
E-mail: industry@diamond.ac.uk
Small angle X-ray scattering (SAXS) from biological macromolecules such as proteins (also called bioSAXS) gives information on the size and shape of macromolecules in solution and can provide details of the radius of gyration and molecular weight of a protein complex (e.g. multi sub-unit complexes), significant conformational changes upon ligand binding, screening of structural changes with formulation conditions (e.g. pH, ionic strength, additives, temperature etc) and many more applications.
At Diamond we offer a full data collection and analysis service for bioSAXS so please do contact us and let us know if you think any of your projects might benefit from SAXS investigations.
On Day 1, talks from synchrotron staff will introduce the technique, the wide variety of applications and data processing, analysis and modelling. Talks from experienced users will provide case study examples of how bioSAXS can be used in a range of industrial and academic research projects.
The second day of the workshop will be dedicated to gaining hands-on experience of the technique through a series of practical sessions focusing on optimum sample preparation, data collection on the beamline, data reduction and data analysis methods.
To allow full participation in the hands-on practical sessions, the number of delegates will be restricted to 30. In the event of over-subscription of the event, we reserve the right to limit the number of participants per company.
The event is free to attend and online registration is now open.
Flexible proteins:
Macromolecular complexes
Ligand binding
Diamond has a dedicated solution state Small Angle X-ray Scattering (SAXS) beamline that can accommodate a wide variety of aqueous-based and some limited organic solvent-based samples. In order to achieve high sample through put, B21 is equipped with a highly automated BIOSAXS robot for small volume liquid handling from a 96 well plate.
B21 also offers Size-Exclusion Chromatography (SEC) coupled SAXS with either Superdex or Shodex SEC columns controlled by an Agilent HPLC. SAXS samples are measured through a temperature controlled capillary. The beamline users benefit from a fast streamlined data processing and data analysis pipeline so data can be analysed during the experiment.
If you are interested in further information about bioSAXS experiments at Diamond, please do contact us by e-mail or phone on 01235 778797.
Further technical information is also available to download including some examples of bioSAXS case studies or why not come and gain some hands on experience of the technique and data processing and analysis at our free workshop in June?
The COVID-19 virus pandemic caused by the SARS-CoV-2 coronavirus poses an ongoing serious global health threat that requires effective and safe therapeutics. The SARS-CoV-2 coronavirus uses its spike glycoprotein to bind to the host receptor angiotensin-converting enzyme-2 (ACE-2) and enter the body’s cells.
The receptor-binding domain (RBD) of the spike glycoprotein is the main target for neutralising antibodies as it can block the virus-host interaction and prevent the infection. The RBD is highly variable and can mutate to escape recognition by antibodies, thus reducing their efficacy and increasing the risk of resistance.
Moreover, the RBD is only exposed transiently on the spike glycoprotein, making it difficult for antibodies to access and bind to it. Novel antibodies are needed that can recognise and neutralise the RBD of SARS-CoV-2 with high potency and stability, regardless of its variability and accessibility
Read more...The ability to modulate drug delivery at therapeutically effective doses over a sustained period of time, in vivo, is very challenging. In the case of poorly water-soluble drugs this requires a carefully designed matrix to manage and maintain their controlled release.
Lipid cubic phase carriers offer an effective way to transport both small molecules and larger proteins through oral and parenteral routes (those outside of the digestive tract), as well as local delivery via subcutaneous and intramuscular routes. Complex interactions between the drug and the lipid matrix govern the release profile; for hydrophilic drugs, release can be very fast. The carriers can also be compromised by naturally occurring lipolytic enzymes which act to break down the lipid microstructure.
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A promising technology is based on phase-change materials (PCMs) that absorb or release large amounts of heat when they change state, e.g. from solid to liquid.
PCMs incorporated into building materials could remove excess heat during the day and release it at night, with minimum carbon emissions. One approach in stabilising PCMs for use is nanoscale confinement in core-shell structures.
Medicinal products extracted from biological sources, called biopharmaceuticals or biologics, must be carefully produced to ensure that only high purity active material is generated. Biopharmaceutical manufacturing processes can have an impact on the amount of product-related variants in the final clinical material. Understanding and controlling amounts of these product-related variants is a major challenge in the development of biopharmaceutical products.
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Read more...Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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