Diamond Annual Review 2019/20

6 7 D I A M O N D L I G H T S O U R C E A N N U A L R E V I E W 2 0 1 9 / 2 0 D I A M O N D L I G H T S O U R C E A N N U A L R E V I E W 2 0 1 9 / 2 0 Diamond’s beamlines: current operational status April 2020 Beamline Name and Number Main Techniques Energy / Wavelength Range Status I02-1 -Versatile MX micro (VMXm) Micro- and nano-focus in vacuum cryo-macromolecular crystallography (VMXm) 7 - 28 keV Commissioning I02-2 -Versatile MX in situ (VMXi) In situ microfocus macromolecular crystallography, Serial Synchrotron Crystallography 10 - 25 keV Commissioning I03 - MX Macromolecular crystallography (MX), Multiwavelength Anomalous Diffraction (MAD) 5 - 25 keV Operational I04 - Microfocus MX MX, MAD 6 - 18 keV Operational I04-1 - Monochromatic MX MX, XChem fragment screening 13.53 keV (fixed wavelength) Operational I05 - ARPES Angle-Resolved PhotoEmission Spectroscopy (ARPES) and nano-ARPES 18 - 240 eV; 500 eV Operational I06 - Nanoscience X-ray Absorption Spectroscopy (XAS), X-ray photoemission microscopy and X-ray magnetic circular and linear dichroism 80eV - 2200eV Operational I07 - Surface and Interface Diffraction Surface X-ray diffraction, Grazing Incidence X-ray Diffraction (GIXD), Grazing Incidence Small Angle X-ray Scattering (GISAXS), X-ray Reflectivity (XRR) 6 - 30 keV Operational B07 -VERSOX:Versatile Soft X-ray Ambient Pressure XPS and NEXAFS 250 - 2800 eV Operational NEXAFS and High-Throughput XPS 50 - 2200 eV Commissioning I08 - Scanning X-ray Microscopy Scanning X-ray microscopy, NEXAFS/ XANES, X-ray fluorescence I08 branch: 250 eV - 4.4 keV Operational J08 - Soft andTender X-ray Ptychography branch: 250 - 2000 eV Construction I09 - Atomic and Electronic Structure of Surfaces and Interfaces XPS (including HAXPES), X-ray StandingWaves (XSW), Near Edge X-ray Absorption Fine Structure (NEXAFS), energy-scanned photoelectron diffraction Hard X-rays: 2.1 - 18+ keV Soft X-rays: 0.1 - 2.1 keV (currently 0.1 - 1.9 keV) Operational I10 - BLADE: Beamline for Advanced Dichroism Experiments Soft X-ray resonant scattering, XAS and X-ray magnetic circular and linear dichroism Circular: 400-1600eV; Linear Horizontal: 250- 1600eV; LinearVertical: 480-1600eV Operational I11 - High Resolution Powder Diffraction X-ray powder diffraction 6 - 25keV (0.5 - 2.1 Å) Operational DIAD: Dual Imaging and Diffraction Simultaneous imaging and diffraction 8 - 38 keV Construction I12 - JEEP: Joint Engineering, Environmental and Processing Time-resolved imaging and tomography (phase- and attenuation-contrast), time-resolved powder diffraction, single crystal diffraction, diffuse scattering, energy dispersive X-ray diffraction (EDXD), high-energy small angle X-ray scattering (under development) 53 keV - 150 keV monochromatic or continuous white beam Operational I13 - X-ray Imaging and Coherence Phase contrast imaging, tomography, full-field microscopy (under commissioning), coherent diffraction and imaging (CXRD,CDI), ptychography and photocorrelation spectroscopy (XPCS) (under commissioning), innovative microscopy and imaging Imaging branch: 8 - 30keV Operational Coherence branch: 7 - 20keV I14 - Hard X-ray Nanoprobe Scanning X-ray fluorescence, X-ray spectroscopy, ptychography and transmission diffraction 5 - 23 keV Operational I15 - Extreme Conditions Powder diffraction, single crystal diffraction Monochromatic and focused 20 - 80 keV White beam Operational I15-1 - XPDF X-ray Pair Distribution Function (XPDF) 40, 65, and 76 keV Operational I16 - Materials and Magnetism Resonant and magnetic single crystal diffraction, fundamental X-ray physics 2.5 - 15 keV Operational B16 -Test beamline Diffraction, imaging and tomography, topography, reflectometry 4 - 20 keV monochromatic focused 4 - 45 keV monochromatic unfocused White beam Operational I18 - Microfocus Spectroscopy Micro XAS, micro Extended X-ray Absorption Fine Structure (EXAFS), micro fluorescence tomography, micro XRD 2.05 - 20.5 keV Operational B18 - Core XAS X-ray Absorption Spectroscopy (XAS) 2.05 - 35 keV Operational I19 - Small-Molecule Single- Crystal Diffraction Small-molecule single-crystal diffraction 5 to 25 keV / 0.5 to 2.5 Å Operational I20 - LOLA:Versatile X-ray Spectroscopy X-ray Absorption Spectroscopy (XAS), X-ray Emission Spectroscopy (XES) and Energy Dispersive EXAFS (EDE) Dispersive branch: 6 - 26 keV Optimisation Scanning branch: 4 - 20 keV Operational I21 - Inelastic X-ray Scattering Resonant Inelastic X-ray Scattering (RIXS), X-ray Absorption Spectroscopy (XAS) Currently 250 - 1500 eV (to be upgraded to 250 - 3000 eV) Optimisation B21 - HighThroughput SAXS BioSAXS, solution state small angle X-ray scattering 8 - 15 keV (set to 13.1 keV by default) Operational I22 - Small Angle Scattering and Diffraction Small angle X-ray scattering and diffraction: SAXS,WAXS, USAXS, GISAXS. Micro-focus. 7 - 20 keV Operational B22 - MIRIAM: Multimode InfraRed Imaging And Mircrospectroscopy IR micro- & nano-spectroscopy THz spectroscopy IR imaging (under commissioning) nanoFTIR : 4000-900 cm -1 (2.5-11 µ m) microFTIR: 10,000-100 cm -1 (1-100 µ m) Spectroscopy (FTIR):10,000-10 cm -1 (1-1000 µ m) Imaging (FPA): 10,000-900 cm -1 (1-11 µ m) Operational I23 - LongWavelength MX Long wavelength macromolecular crystallography 3 - 8 keV (1.5 - 4.1 Å) Optimisation B23 - Circular Dichroism Circular Dichroism (CD) 125-500 nm& 165-650 nm for CD Imaging at 50 µm resolution, 96-cell High-Throughput CD (HTCD) and High-Pressure CD up to 200 MPa Operational I24 - Microfocus and Serial MX Macromolecular crystallography, MAD, Serial Crystallography 6.5 - 25.0 keV Operational B24 - CryoTransmission X-ray Microscopy (TXM) Full field X-ray imaging 200eV - 2600eV Optimisation Krios I Krios IV Krios V Glacios Aquilos ARM 200F ARM 300F Talos Arctica Scios Krios II Krios III I04 Microfocus MX I05 ARPES: Angle-Resolved Photoemission Spectroscopy I06 Nanoscience I07 Surface and Interface Di raction B07 VERSOX: Versatile Soft X-ray I08 Scanning X-ray Microscopy I09 Atomic and Electronic Structure of Surfaces and Interfaces I10 BLADE: X-ray Dichroism and Scattering I11 High Resolution Powder Di raction & Long Duration Experiments (LDE) I12 JEEP: Joint Engineering, Environmental and Processing DIAD: Dual Imaging and Di raction I13 X-ray Imaging and Coherence Hard X-ray Nanoprobe I14 XPDF: X-ray Pair Distribution Function I15-1 Materials and Magnetism I16 B16 Microfocus Spectroscopy I18 Core XAS: X-ray Absorption Spectroscopy B18 Small-Molecule Single-Crystal Di raction I19 LOLA: Versatile X-ray Spectroscopy I20 Inelastic X-ray Scattering I21 High Throughput SAXS B21 Small Angle Scattering and Di raction I22 MIRIAM: Infrared Microspectroscopy B22 LongWavelength MX I23 Circular Dichroism B23 Microfocus and Serial MX I24 I02-2 VMXi: Versatile MX in situ I02-1 VMXm: Versatile MX micro Cryo-TXM: Cryo-Transmission X-ray Microscopy B24 Membrane Protein Laboratory I03 MX I04-1 MX Extreme Conditions I15 XChem facility for fragment-based screening UK X-ray Free Electron Laser (XFEL) Hub electron Physical Science Imaging Centre (ePSIC) electron Bio-Imaging Centre (eBIC) Test Beamline Microscope Main Capabilities Accelerating Voltages Operational Status Titan Krios I Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2015 Titan Krios II Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2016 Titan Krios III Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2017 Titan Krios IV Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2017 Titan Krios V Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2018 Talos Arctica Cryo-EM, Cryo-ET 200 kV Operational since 2016 Glacios Cryo-EM, Cryo-ET 200 kV Installed, March 2019 Scios Cryo-SEM, Cryo-FIB 3 to 30 kV Operational since 2017 Aquilos Cryo-SEM, Cryo-FIB 3 to 30 kV Operational since 2019 JEOL ARM200F Atomic scale STEM imaging, EELS, EDX, electron diffraction 80, 200 kV Operational since 2017 JEOL ARM300F Atomic scale TEM and STEM imaging, electron diffraction, 4D-STEM, EDX 30, 60, 80, 160, 200, 300 kV Operational since 2017 Electron Microscopes I n its thirteenth year of experiments, Diamond is now operating with 32 beamlines and 11 electron microscopes. This year saw the completion of the latest phase of construction and the DIAD beamline will hopefully welcome first users in early 2021. Of the electron microscopes, nine are cryo-electron microscopes specialising in life sciences and make up eBIC (electron Bio-Imaging Centre), with two provided for industry use in partnership with Thermo Fisher Scientific. The two remaining microscopes dedicated to advanced materials research are supplied by Johnson Matthey and the University of Oxford. These microscopes form ePSIC (electron Physical Science Imaging Centre) and are operated under strategic collaboration agreements to provide for substantial dedicated peer reviewed user access. Both eBIC and ePSIC are next to the Hard X-ray Nanoprobe beamline (I14). Along with eBIC and ePSIC, the UK X-ray Free Electron Laser (XFEL) Hub, the Membrane Protein Laboratory and the XChem facility make up the complimentary integrated facilities available at Diamond. For academic research, Diamond instruments (beamlines and microscopes) are free at the point of access through peer review. For proprietary research, access can be secured through Diamond’s industry team. Following a restructure and subsequent internal changes, the instruments and beamlines are organised into eight science groups as described below. Beamline Development and Technical Summary Macromolecular Crystallography Soft Condensed Matter Biological Cryo-Imaging Spectroscopy Magnetic Materials Crystallography Imaging and Microscopy Structures and Surfaces