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Please see below for details of the current User Programme Calls from eBIC.
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In collaboration Rosalind Franklin Institute & Diamond are pleased to announce a commissioning call for researchers to access the cryo volume imaging capabilities of the Helios Hydra cryoPFIB/SEM housed at Diamond.
The Helios Hydra is the latest generation plasma FIB/SEM from ThermoFisher and has some unique capabilities Helios Hydra 5 DualBeam:
These capabilities are being developed through a tripartite collaboration between the Rosalind Franklin Institute, Diamond and ThermoFisher Scientific funded by the Wellcome Trust and EPSRC. This call aims to provide a platform for the wider user community to experience serial cryoPFIB/SEM volume imaging on the Helios Hydra (Dumoux, 2022) and will help inform the collaboration partners on the wider user requirements/desire for this type of experiment in the future.
Please select the Rapid access route and Helios instrument when you submit your proposal.
Proposals are also open to existing BAG PIs.
Proposals will be reviewed by eBIC staff (the Rosalind Franklin Institute has no part in the review process) with a view to schedule beamtime from August/September 2023.
Download the PDF for more details on requirements and visit structure: Helios Hydra call.
We are pleased to announce a commissioning call for Cryogenic Correlated Light and Electron Microscopy (Cryo-CLEM) proposals, at eBIC.
Cryo-light and electron microscopy can be used to image and correlate samples in frozen hydrated specimen in a close-to-native state to achieve high-resolution structural data.
Submit your proposal
• For lamella preparation on frozen cells and microcrystals
• On-grid FIB milling only
• Rapid and BAG access
For more information please see here (download).
We are pleased to announce a commissioning call for researchers to access the chameleon cryo-EM freezing device (application) developed commercially by SPT Labtech. This forms part of a collaboration between Diamond, the Rosalind Franklin Institute and SPT Labtech.
The chameleon uses piezo electric inkjet dispensing technology to apply nanolitre volumes of protein solution to self-wicking cryo-EM grids.
Please select the Rapid access route and the Talos instrument when you submit your proposal and also add that this is specifically for use with the chameleon.
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