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The scattering intensity of a studied system is given by:
|I(s)||Scattering intensity of the sample|
|Is(s)||Scattering intensity of the solution|
|Ib(s)||Scattering intensity of the background|
|Is,0||Intensity of direct beam through the sample|
|Ib,0||Intensity of direct beam through the blank sample (e.g. buffer only)|
|c||Concentration of the sample|
The resolution of SAXS is about 15 Å.
Four requirements must be fulfilled before performing a SAXS experiment:
The scattering intensity of the studied system is obtained by subtracting the background due to parasitic scattering from the slits and/or the windows of the sample holder. In case of molecules in a buffer, the buffer must also be subtracted. This is done by measuring a blank sample, e.g. a sample containing all the components except the molecule of interest.
The intensity of the direct beam through the sample is measured by a photodiode in the beamstop. This is collected automatically, and used to normalise the scattering data. The intensity of the incoming beam is monitored by a beam position monitor just upstream of the sample position.
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