Email: david.hall@diamond.ac.uk
Tel: +44 (0) 1235 778926
Establishing ideal conditions for freezing membrane protein samples can be time consuming. The MPL can support visiting scientists to establish membrane protein cryo-EM based projects. With access to the RCaH Glacios with Falcon 4 detector and JOEL JEM-2100 TEMs we have established a screening pipeline that enables sample characterisation to support direct eBIC access through the eBIC user access programme.
These microscopes are supported by two vitrobots and a Leica GP2 for grid preparation. A gradient maker is also avalible which is useful for the GraFix1 and GraDeR2 approaches to preparing samples for cryo-EM.
1. Kastner B, Fischer N, Golas MM, Sander B, Dube P, Boehringer D, Hartmuth K, Deckert J, Hauer F, Wolf E, Uchtenhagen H, Urlaub H, Herzog F, Peters JM, Poerschke D, Lührmann R, Stark H. GraFix: sample preparation for single-particle electron cryomicroscopy. Nat Methods. 2008 Jan;5(1):53-5. doi: 10.1038/nmeth1139. Epub 2007 Dec 23. PMID: 18157137.
2. Hauer F, Gerle C, Fischer N, Oshima A, Shinzawa-Itoh K, Shimada S, Yokoyama K, Fujiyoshi Y, Stark H. GraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM. Structure. 2015 Sep 1;23(9):1769-1775. doi: 10.1016/j.str.2015.06.029. Epub 2015 Aug 13. PMID: 26278176.
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Macromolecular Crystallography
Macromolecular
Soft Condensed 
Imaging and
Biological
Magnetic
Structures and
Crystallography 
Spectroscopy 
