Cryo Electron Microscopy in Structural Biology

To provide fundamental and practical lectures in cryo-EM, from the electron microscope and sample preparation, through to image formation and processing, reconstruction in SPA and cryo-ET, and model building.

This course is a hybrid event targeted at the Oxford Wellcome Trust Doctoral Training Program (WT DTP) students but lectures made open to the wider Harwell campus community via a virtual platform. Practical sessions cover sample preparation, cryo-EM screening, SPA data collection and cryo-ET.

Where possible the slides and recordings of the lecturers are provided for download in the programme below.

Day 1: Tuesday, August 16th 2022

Lecture 1: Why cryogenic electron microscopy (cryo-EM)? – Kyle Morris (eBIC)
Lecture 2: The electron microscope – Karen Davies (eBIC)
Lecture 3: Sample preparation – Dimple Karia (Thermo Fisher Scientific)
Practical 1: Cryogenic sample preparation for cryo-EM – Peter Harrison (eBIC)

Day 2: Wednesday, August 17th 2022

Lecture 4: Image formation in the electron microscope – Dan Clare (eBIC)
Lecture 5: Image processing – Colin Palmer (CCP-EM)
Lecture 6: From 2D images to 3D density maps in SPA – Yuriy Chaban (eBIC)
Practical 2: Screening samples in cryo-EM – Peter Harrison (eBIC)

Day 3: Thursday, August 18th 2022

Lecture 7: From 2D images to 3D density maps in cryo-ET – Lindsay Baker (Oxford)
Lecture 8: Averaging and reconstructing 3D maps in cryo-ET – Pranav Shah (Oxford)
Lecture 9: Fitting and building of atomic models – Agnel-Praveen Joseph (CCP-EM)
Practical 3: Single-Particle Averaging data collection demonstration – Vinod Vogirala (eBIC)

Day 4: Friday, August 19th 2022

Practical 4: Cryo-ET data collection demonstration – Davide Zabeo (eBIC)
Research talks by eBIC PDRA & PhD students

Download full programme