Becoming an ‘independent’ single particle data collector

Feb 21. till Feb 24.

Becoming an ‘independent’ single particle data collector

We are pleased to announce this 4-day virtual course as part of the community-led training program in Cryo-EM funded by the Wellcome Trust and MRC.

The course, jointly organised between the Diamond Light Source and the University Leeds, aims to help attendees gain independence in Single Particle Analysis data acquisition and learn tips and tricks for their own cryo-EM instrumentation and experiments.

Date
21/02/20222022-02-21T10:00:00 - 24/02/20222022-02-24T16:30:00
Time
10:00 - 16:30
Location
Online via Zoom
Link
Apply here

Overview

The purpose of this course is to provide training to scientists working in Cryo-EM for how to perform Single Particle Analysis data collection and analysis.

Specifically the course is targeted at those users who already have some experience with data collection and are looking to gain further independence. 

During the course the Diamond Light Source eBIC: Electron Bio-Imaging Centre and the University of Leeds ABSL will provide remote access to their instrumentations.

The first day will be dedicated to the demonstration of the TFS EPU software for setting up single particle acquisition by an expert instructor. This will be followed by two days where the control will be given to participants, who will be guided through the processes previously observed. The final day will be a session dedicated to data analysis, with a focus on handling data from on-the-fly analysis pipelines.

 

Programme

Download the full programme here.

Monday 21st February: 

  • Protocol demonstration.
  • Set up of single particle data acquisition in EPU on a Titan Krios microscope.

Tuesday 22nd and Wednesday 23rd February: 

  • Hands-on practicals in small groups: attendees will perform remote set up with instructor support.
  • Additional opportunity for observation and questions, to reinforce learning.

Thursday 24th February

  • Introduction to image processing concepts, real-time analysis and strategies for refinement after data collection.

Further info & Registration

This online event is free to attend but spaces are limited and will be assigned upon application.

Applications is now open until the end of Sunday 30th January 2022.

All applicants will be informed by Monday 7th February 2022 on the outcome of their applications. 

APPLY HERE

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

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