Instruct UK CryoEM Sample Preparation Workshop

Mar 24. till Mar 27.

Instruct UK CryoEM Sample Preparation Workshop

The goal of this workshop is to enable cryoEM users to improve their skills and knowledge in the preparation of samples for imaging on cryo electron microscopes, primarily through hands-on tutorials, discussions and lectures.

Date
24/03/20202020-03-24T09:00:00 - 27/03/20202020-03-27T17:00:00
Time
09:00 - 17:00
Location
Diamond Light Source

Overview

This workshop is organised in collaboration with:
 
                           
 
 
The goal of the workshop is to teach users the best practices in sample preparation for cryoEM imaging. The workshop will be principally hands-on with students preparing vitrified samples on the latest generation of plunge-freezing devices. Grid modification techniques and clipping and loading for Thermo Fisher Scientific autoloaders will also be taught.  Students will screen the samples they freeze with the eBIC Titan Krios & Talos Arctica microscopes.
 
This workshop will be geared toward researchers relatively new to the field of cryoEM, but who already have some experience of plunge freezing.  The course is only open to academic applicants.  The course is funded by Instruct & Diamond Light Source.  This funding includes the cost of the course, accommodation and subsidence.  However, you will need to cover the cost of your travel to and from the course. 
 
Applicants must show they are using cryoEM as part of their research and have access to a plunge-freezing device. The workshop aims to equip participants with the necessary skill set to further develop their cryoEM expertise at their home laboratories and eBIC. Several biological test samples will be provided allowing participants hands on experience with a variety of samples.

Application process for academic participants

Places are strictly limited to 12 delegates. Places will be allocated on the grounds of merit and anticipated benefit. 

All applicants must meet the following criteria;
You are using cryoEM as part of your current research and already have some experience.
You have access to a plunge freezing device.
 
Before beginning the application process please prepare the following documents to upload to the form;
A letter describing your motivation for applying for the course, and how this will benefit your research and/or facility.  Please also describe your current role/position and your groups plans for using EM.
A letter of support from your PhD supervisor or lab PI if you are a PostDoc.
 
 
KEY DATES
Applications close – 10th February 2020
All applicants will be notified with the outcome of their application by 2nd March 2020.
 
If successful, you will be asked to formally accept your place by registering online. Your place on the course will be free of charge and will include:
- all meals and refreshments 
- accommodation at Ridgeway House based on Harwell Campus, checking in on Tuesday 24th March and checking out on Friday 27th March.
You will need to arrange your own travel to get to Diamond however, and this will not be covered by Diamond.

Draft agenda

Tuesday 24th March
13:30–14:30 Registration
14:30 Welcome (Katie Cunnea)
14:35 Health and Safety talk
14:45–15:15 Tours of eBIC & Diamond
15:30–17:00 CryoEM sample prep tutorial & group exercise
 
 
Wednesday 25th March
09:00–12:00 Practical session 1
12:00 Lunch
12:30–13:15 Lecture: CryoEM sample preparation for ‘difficult’ samples - Rebecca Thompson, Astbury Centre, University of Leeds
13:15–13:45 Lecture: Future technology for freezing cryoEM samples
14:00–17:00 Practical session 2
17:00 Wrap up session 
18:30 Dinner
 
Thursday 26th March
09:00–12:00 Practical session 3
12:00 Lunch
12:30–13:15 Lecture: Single particle analysis data collection challenges - Katie Cunnea, eBIC
13:15–13:45 Lecture: Time resolved cryoEM - David Klebl, Astbury Centre, University of Leeds
14:00–17:00 Practical session 4
17:00 Wrap up session 
18:30 Dinner
 
Friday 27th March
09:00 Practical Session 5
12:00 Lunch
12:30 Lecture: Latest advances in cryoET
13:30 Group photograph 
13:45 Student feedback presentations 
15:45 Closing remarks 
16:00 Course End and Depart
 
*this agenda is subject to change

Contact

 For any further information about this workshop, please contact the Diamond Events team.

 

Parking on campus

 Due to a large number of building works on site, parking on the Harwell Campus is extremely limited and visitors’ parking cannot be guaranteed. We strongly recommend travelling by public transport, where possible, if you are attending an event at Diamond. We apologise for any inconvenience caused. Find here more information on how to get to Diamond by public transport.