MicroED Workshop

Nov 06. till Nov 08.

Electron diffraction method for cryo-electron microscopes

The goal of this workshop is to introduce MicroED to structural biologists. The course will consist of lectures from microED leading experts Tamir Gonen, Xiadong Zou, David Waterman and Abhay Kotecha. Data acquisition will be taught using EPU-D on Talos/Krios cryo-electron microscopes and participants will be shown how to reduce/process data and solve structures.

Date
06/11/20192019-11-06 - 08/11/20192019-11-08
Location
eBIC, Diamond Light Source

About the workshop

The goal of this workshop is to teach users the electron diffraction method in cryo-electron microscopes, from setting up the microscope for diffraction data collection to image processing. The workshop will be principally hands-on with students working on the Talos Arctica, and/or Krios, microscopes, as well as image processing tutorials.


This workshop is available to academics only and is geared toward experienced structural biologists wanting to learn MicroED.  The course is sponsored by Thermo Fisher Scientific and Diamond Light Source and is free to academic delegates (includes food and accommodation).  

Applicants must show they plan to use electron diffraction as part of their research and have access to appropriate equipment to do so. The workshop aims to equip participants with the necessary skill set to further develop their cryo-EM expertise in diffraction at their home laboratories and eBIC. Several biological test samples will be provided allowing participants hands on experience with both protein and small molecule crystals.  Applicants can also request to bring pre-screened (pre-clipped) grids to look at on the last day of the course.

Topics

Hands-on Sessions

  • MicroED data acquisition on the Talos Artica or Krios
  • Image processing of MicroED data with DIALS
  • Demonstration of Aquilos lamella preparation of frozen-hydrated crystals
     

How to Apply

Places are strictly limited to 12 delegates. Places will be allocated on the grounds of merit and anticipated benefit.

All applicants must meet the following criteria:

  • Have previous experience of X-ray crystallography data collection and processing or cryo-EM data collection and processing.
  • Show how they plan to use the MicroED method in their research.

Before beginning the application process please prepare the following documents to upload to the form:

  • A letter describing your motivation for applying for the course, and how this will benefit your research and/or facility.  Please also describe your current role/position and your groups plans for using MicroED.
  • A letter of support from your PhD supervisor or lab PI if you are a PostDoc.

APPLICATIONS ARE NOW CLOSED

Key Dates & Enquiries

Applications close Monday 30th September 2019 at 23:30 (BST)
Notification of application result By Friday 4th October 2019
Successful applicants' registration deadline Monday 28th October 2019
Workshop opens Wednesday 6th November 2019
Workshop closes Friday 8th November 2019

 

Please direct any enquiries to katie.cunnea@diamond.ac.uk

Outline Progamme

Wednesday 6th November 2019

08:30 - 09:00 Registration, Tea/Coffee
09:00 - 10:30 Welcome & Lectures
10:30 - 11:00 Tea/Coffee
11:00 - 13:00 Practical session 1
13:00 - 14:00 Lunch
14:00 - 15:30 Practical session 2
15:30 - 15:50 Tea/Coffee
15:50 - 17:00 Practical session 2 cont.
17:00 - 18:30 Free time
18:30 Dinner

 
Thursday 7th November 2019

09:00 - 10:30 Lectures
10:30 - 10:50 Tea/Coffee
11:00 - 13:00 Practical session 1
13:00 - 14:00 Lunch
14:00 - 15:30 Practical session 2
15:30 - 15:50 Tea/Coffee
15:50 - 17:00 Practical session 2 cont.
17:00 - 18:30 Free time
18:30 Dinner


Friday 8th November 2019

09:00 - 12:00 Aquilos demonstration
12:00 - 12:45 Lunch
12:45 DEPART

 

Parking on Campus

Due to a large number of building works on site, parking on the Harwell Campus is extremely limited and visitors’ parking cannot be guaranteed. We strongly recommend travelling by public transport, where possible, if you are attending an event at Diamond. We apologise for any inconvenience caused. Find here more information on how to get to Diamond by public transport.