Micro-ED Course

Nov 06. till Nov 08.

Micro-ED Course

This course took place in November 2019, and goal was to teach users the electron diffraction method in cryo-electron microscopes, from setting up the microscope for diffraction data collection to image processing.

Date
06/11/20192019-11-06T09:00:00 - 08/11/20192019-11-08T16:30:00
Time
09:00 - 16:30
Location
Diamond Light Source, Oxfordshire, UK

Past Course: 6th-8th November 2019, Electron Diffraction

The goal of this workshop was to teach users the electron diffraction method in cryo-electron
microscopes, from setting up the microscope for diffraction data collection to image processing.

The workshop was hands-on with students working on the Talos Arctica, and/or Krios, microscopes,
as well as image processing tutorials.

 Hands-on Sessions:

  • MicroED data acquisition on the Talos Artica or Krios
  • Image processing of MicroED data with DIALS
  • Demo of Aquilos lamella preparation of frozen-hydrated crystals     

Please find a couple of video's and presentations from the course below.

Lecture 1: Dials for ED (Data Analysis and structure determination using DIALS and CCP4)

Presenter: David Waterman - STFC
Slides

 

 

Lecture 2: Micro-Electron Diffraction, Single Particle Analysis and Cryo-Electron Tomography:

A hybrid approach for structure determination

Presenter: Abhay Kotecha - Thermo Fisher Scientific

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

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