Cryo-EM Sample Preparation Workshop

Oct 02. till Oct 04.

Cryo-EM Sample Preparation Workshop

The goal of this workshop is to teach new CryoEM users how to prepare samples for imaging on the cryo electron microscope, primarily through hands-on tutorials, discussions and introductory talks.

Date
02/10/20182018-10-02T09:00:00 - 04/10/20182018-10-04T17:00:00
Time
09:00 - 17:00
Location
Diamond Light Source

Overview

The goal of the workshop is to teach users the best practices in sample preparation for cryo-EM imaging. The workshop will be principally hands-on with students preparing vitrified samples on the latest generation of plunge-freezing devices.  They will then have the opportunity to subsequently screen these samples with the eBIC Titan Krios and Talos Arctica electron microscopes.

This workshop will be geared toward novices with the only requirement being that applicants show they plan to use cryo-EM as part of their current research and have access to a plunge-freezing device. The workshop aims to equip participants with the necessary skill set to further develop their cryo-EM expertise at their home laboratories. Several biological test samples will be provided allowing participants hands on experience with a variety of samples.
 

Topics

Introductory Lectures:

  • An introduction to the basics of cryo-EM and cryo-ET
  • The complete guide to sample vitrification 

Hands-on sessions:

  • Sample preparation for single particle cryo-EM
  • Sample screening at eBIC and an introduction to data collection on Titan Krios and Talos Arctica

Industry Presentations:  

Presentions will be given on the next generation of sample preparation equipment

  • TTP Labtech - Spot-it-on
  • Cryosol - VitroJet

How to apply

The workshop is free to attend (including accommodation and catering), but places are strictly limited to 12. Places will be allocated on the grounds of merit and anticipated benefit.

All applicants must meet the following criteria;

  • You plan to use cryo-EM as part of your current research
  • You have access to a freeze plunging device

Before beginnng the application process please prepare the following documents to upload to the form;

  • Your CV/Resume including a description of your experience, other skills
  • A separate statement (up to 150 words) explaining why you want to apply to this course and the relevance to your current research
  • A letter of support from your PhD supervisor or lab PI if you are a PostDoc

APPLY HERE 

KEY DATES

Applications close - 15th August 2018
All applicants will be notified with the outcome of their application - 24th August

ENQUIRIES

events@diamond.ac.uk

 

Provisional Programme

 

 

Monday 1st October

17:00

Arrival of course participants and registration

 

Tuesday 2nd October

08:30

All you need to know about Cryo-EM sample preparation

09:30

Coffee Break

09:50

Health and safety lab inductions

10:00

Practical Session I

12:45

Lunch

13:30

Discussion Session

14:00

Practical Session II (inc. coffee break)

17:00

Wrap up session and feedback day 1

18:00

Group Dinner

 

Wednesday 3rd October

08:30

All you need to know about Cryo-EM

09:30

Coffee Break

09:50

Practical Session III

12:45

Lunch

13:30

Discussion Session

14:00

Practical Session IV (inc. coffee break)

17:00

Wrap up session and feedback day 2

18:00

Group Dinner

 

Thursday 4th October

08:30

Intro to Cryo and Correlative techniques

09:30

Coffee Break

09:50

Practical Session V

12:45

Lunch

13:30

Discussion Session

14:00

Practical Session VI (inc. coffee break)

15:30

Student Presentations

16:45

Course end and depart

 

 

Course Instructors

  • Dan Clare
  • Yuriy Chaban
  • Andy Howe
  • James Gilchrist
  • Katie Cunnea
  • Zhengyi Yang
  • Adriana Klyszejko
  • Rebecca Thompson
  • Jan Weert
  • Natasha Lukoyanova
  • Cornelia Cazey
     

 

Venue

The course is residential, with accommodation provided locally. 

Lectures and tutorials will take place at Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE. 

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

Copyright © 2020 Diamond Light Source

 

Diamond Light Source Ltd
Diamond House
Harwell Science & Innovation Campus
Didcot
Oxfordshire
OX11 0DE

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