B23 Roadshow Series - nucleic acid research

Feb 11. till Feb 11.

B23 Roadshow Series - nucleic acid research

Diamond Light Source (DLS) together with the University of Southampton held a one-day workshop dedicated to nucleic acid research, with a particular view to promote the synchrotron based CD beamline B23 for bio-nanomaterials.

Date: 11/02/20132013-02-11 - 11/02/20132013-02-11

Location: The Winchester Hotel, Winchester

On the 11th February 2013, the Diamond Light Source (DLS) together with the University of Southampton held a one-day workshop dedicated to nucleic acid research, with a particular view to promote the synchrotron based CD beamline B23 for bio-nanomaterials. The event was held at The Winchester Hotel in a historic city of Winchester, a convenient location well accessible using public transport.

The day included scientific lectures about bio-nanomaterials, use of CD spectroscopy in selected applications and other biophysical techniques, and an introduction to the B23 station from the beamline scientists. Part of the day was devoted to the advantages of B23 over bench-top instruments, specific experimental setup, possibilities and limitations, and to the peer reviewed beamtime proposal process. B23 is – like all other beamline stations at DLS – accessible to UK academics through the online proposal submission system, and the visits are fully supported by DLS.
Registration for this event is now closed.
This was the first event in a series of Road shows to highlight Diamond Light Source B23 throughout the country. The next events are scheduled to take place in Leeds on the 31st May 2013 and in Liverpool on the 1st November 2013. If you are interested in participating in future events please please send an email to either Eugen Stulz or to Rohanah Hussain.

Programme

11 February 2013, The Winchester Hotel
Diamond Light Source – University of Southampton

09:30 Registration

09:55 Welcome note

10:00 B23 overview and update

10:30 CD Spectroscopy as a tool to analyse modified DNA
Eugen Stulz, Southampton University

11:00 Exploring the role of lipid/protein interactions in cellular processes by circular dichroism
Phil Williamson, Southampton University

11:30 Coffee/Tea break

12:00 DNA-response regulator protein interactions: insights into the DNA binding specificity of the effector domain of the global regulator PrrA (RegA) from Rhodobacter sphaeroides PrrA
Mary Phillips-Jones, University of Central Lancashire

12:30 Unpair to Repair at DNA and RNA Duplex Termini
David Finger, Sheffield University

13:00 Lunch

14:00 Using CD to investigate the role of nucleic acid structure in gene regulation
Garry Scarlett, Portsmouth University

14:30 Probing DNA conformation and DNA-enzyme interaction using time-resolved fluorescence
Anita Jones, Edinburgh University

15:00 Use of the 3’-S phosphorothiolate substitution to modify DNA structure, as determined
by NMR spectroscopy and UV thermal melting studies
Julie Fisher, University of Leeds

15:30 Coffee break

16:00 Synthesis and Study of Modified Photochromic and Organometallic nucleic acids
Jim Tucker, Birmingham University

16:30 Feedback and Discussion

17:00 End of Workshop