Applications for this project are now closed.
A recent study on NAD/FAD-dependent ketoreductases found that shining light of a specific wavelength on these enzymes bestowed upon them the ability to break one of the strongest chemical bonds in nature, the ability to perform dehalogenation. A phenomenon now known as photoinduced promiscuity. This project aims to structurally characterise photoinduced promiscuity by performing time-resolved X-ray crystallography experiments on these ketoreductase enzymes.
Initially this project will be focused on wet lab work, expressing, purifying, and crystallising ketoreductases in the Research Complex at Harwell. This process will likely take up the bulk of the placement. Once the proteins have been successfully crystallised the student will learn how to collect and process diffraction data on Beamline I24 to solve macromolecular structures, possibly using laser activation to determine different conformations brought about through photoinduction. If this work can be complete ahead of schedule the student will begin development on a protocol to produce microcrystals, which can be used to perform time-resolved structural studies and make molecular movies of the chemical process.
Please apply via our online application portal. The vacancy that you are applying for is the "Summer Placements 2022" listing, you will then have the opportunity to select up to three projects to apply for. This project's reference is 22003SP.
Applications are now closed with interviews scheduled for 7, 8, 9 and 10 February 2022.
If you are disabled and would like to be considered under the Guaranteed Interview Scheme, please let us know via the online application process.
Please note that this role does not meet the required skill level for a Skilled Worker visa and therefore we would be unable to sponsor individuals due to the current UK Home Office immigration rules. To be appointed to the role, candidates will need to have the right to work in the UK without sponsorship from us.
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