The first automated data set of a test specimen, GroEL, has been successfully collected on Diamond's cryo-electron microscope, Titan Krios I.
The data set was collected with the world's first EPU embedded bioquantum-K2 detector, and was produced in the form of films so that researchers could see a moving image of the specimen. The films were collected in super-resolution counting mode with a 30eV energy selecting slit.
The films were then aligned using IMOD followed by particle selection, alignment and 3D reconstriuction in relion. From a total of 30,000 particles, 17,500 were used to calculate a 3.2Å 3D reconstruction of GroEL. This is currently the highest resolution 3D reconstruction of GroEL determined by cryo-electron microscopy - a significant achievement for Diamond's Titan Krios I.
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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