Corey Hecksel

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Corey is an EM Scientist for the electron Bio-Imaging Centre (eBIC). He joined in May 2016 from the Baylor College of Medicine.

Email: corey.hecksel@diamond.ac.uk
Tel: +44 (0) 1235 77 8196

Key Research Area

  • Structural biology
  • Virus-host interactions
  1. Research
  2. Publications
Research -

Until recently cryo-electron microscopy (Cryo-EM) has been limited to studying purified macromolecular complexes that are no longer in their native environment, the intact mammalian cell. I am currently working on methods for culturing numerous mammalian cells for cryo-EM as well as direct visualization of protein complexes in these cells.

Using cryo-electron tomography (cryo-ET) in combination with cryo focused ion beam scanning electron microscopy (cryoFIB-SEM) of whole mammalian cells we aim to investigate the structural relationships of biological complexes in their native cellular environment. We aim to provide novel insights into the mechanisms of viral pathogenesis as well as providing a foundation for future biological research.

Publications - +

1. Schmid, M.F., Hecksel, C.W., Rochat, R.H., Bhella, D., Chiu, W., Rixon, F.J. (2012). A tail-like assembly at the portal vertex in intact herpes simplex type-1 virions. PLoS Pathog 8(10): e1002961. PMID23055933


2. Rochat, R.H., Hecksel, C.W., Chiu, W. (2014) Cryo-EM techniques to resolve the structure of HSV-1 capsid-associated components. Methods Mol Biol. PMID: 24671690
 

3. Zhang, S., Sroller, V., Zanwar, P., Chen, C.J., Halcorson, S.J., Ajami, N.J., Hecksel, C.W., Swain, J.L., Wong, C., Sullican, C.S., Butel, J.S. (2014) Viral microRNA effects on pathogenesis of polyomavirus SV40 infections in Syrian golden hamsters. PLoS Pathog 10(2): e1003912. PMID: 24516384
 

4. Chiu W, Hecksel C, & Hryc CF. (2015) Electron Cryo-Microscopy and Cryo-Tomography of Viruses. Reference module in Biomedical Sciences. Elsevier. doi: 10.1016/B978-0-12-801238-3.02553-8.
 

5.Hecksel, C, and Rico-Hesse, R. Arbovirus evolution, vector competence, and virulence models – changing patterns of infection. The Influence of Global Environmental Change on Infectious Disease Dynamics, Washington (DC): National Academies Press (US); 2016.
 

6. Hecksel C, Darrow MC, Dai W, Galaz-Montoya JG, Chin JA, Mitchell PG, Chen S, Jakana J, Schmid MF, Chiu W. (2016) Quantifying Variability of Manual Annotation in Cryo-Electron Tomograms. Microsc Microanal 22(3): 487-96. PMID: 27225525
 

7. Galaz-Montoya JG, Hecksel CW, Baldwin PR, Wang E, Weaver SC, Schmid MF, Ludtke SJ, Chiu W. (2016) Alignment algorithems and per-particle CTF correction for single particle cryo-electron tomography. J Struct Biol. 194(3)383-94. PMID: 27016284