A buffer measurement must be performed before and after each sample. The buffer must be identical to the one diluting the sample.
For each sample it is best practice to measure a concentration series (e.g. 1, 5, 10 mg/ml).
The geometrical structure information is obtained from the scattering intensity normalised to one electron in the solution, which is usually obtained by reference to a standard sample. At the beginning of the run, a standard sample (Lysozyme) at 5mg/ml must be measured. BSA can also be measured but is more sensitive to radiation damage.
A series of 120 frames of 1s exposure ensures the data analysis will be performed on only samples without radiation damage.
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