Jose Trincao

My main research ares have been:

• Protein-DNA/RNA complexes
• Antibiotic resistance in S. aureus
• Metalloenzymes

I am now concentrating on new methods for sample mounting and visualisation to be applied at Diamond, mainly for smaller samples.

Current microfocus beamlines have been pushing the limits of flux and beam size, allowing to collect data on smaller and smaller crystals. As these decrease in size, handling and visualization becomes an issue. We are working on novel methods for mounting protein crystals, borrowing ideas from cryo-EM or developing new ones.

Development of Time-resolved techniques in collaboration with the Dynamic Structural Sciences consortium headed by Prof. Paul Raithby (Bath University).

• Vieira D, Figueiredo TA, Verma A, Sobral RG, Ludovice AM, de Lencastre H, Trincao J. Purification, crystallization and preliminary X-ray diffraction analysis of GatD, a glutamine amidotransferase-like protein from Staphylococcus aureus peptidoglycan. Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):632-5.

• Hatcher LE, Christensen J, Hamilton ML, Trincao J, Allan DR, Warren MR, Clarke IP, Towrie M, Fuertes S, Wilson CC, Woodall CH, Raithby PR. Steady-state and pseudo-steady-state photocrystallographic studies on linkage isomers of [Ni(Et4dien)(η2-O,ON)(η1-NO2)]: identification of a new linkage isomer. Chemistry. 2014 Mar 10;20(11):3128-34.

• Tanner SJ, Ariza A, Richard CA, Kyle HF, Dods RL, Blondot ML, Wu W, Trincão J, Trinh CH, Hiscox JA, Carroll MW, Silman NJ, Eléouët JF, Edwards TA, Barr JN. Crystal structure of the essential transcription antiterminator M2-1 protein of human respiratory syncytial virus and implications of its phosphorylation. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1580-5. doi: 10.1073/pnas.1317262111.

• Trincao J, Hamilton ML, Christensen J, Pearson AR. Dynamic structural science: recent developments in time-resolved spectroscopy and X-ray crystallography. Biochem Soc Trans. 2013 Oct;41(5):1260-4.

• Coelho C, Mahro M, Trincão J, Carvalho AT, Ramos MJ, Terao M, Garattini E, Leimkühler S, Romão MJ. The first mammalian aldehyde oxidase crystal structure: insights into substrate specificity. J Biol Chem. 2012 Nov 23;287(48):40690-702.

• Mahro M, Coelho C, Trincão J, Rodrigues D, Terao M, Garattini E, Saggu M, Lendzian F, Hildebrandt P, Romão MJ, Leimkühler S. Characterization and crystallization of mouse aldehyde oxidase 3: from mouse liver to Escherichia coli heterologous protein expression. Drug Metab Dispos. 2011 Oct;39(10):1939-45.

• Coelho C, González PJ, Moura JG, Moura I, Trincão J, João Romão M. The crystal structure of Cupriavidus necator nitrate reductase in oxidized and partially reduced states. J Mol Biol. 2011 May 20;408(5):932-48.

• Mukhopadhyay A, Kladova AV, Bursakov SA, Gavel OY, Calvete JJ, Shnyrov VL, Moura I, Moura JJ, Romão MJ, Trincão J. Crystal structure of the zinc-, cobalt-, and iron-containing adenylate kinase from Desulfovibrio gigas: a novel metal-containing adenylate kinase from Gram-negative bacteria. J Biol Inorg Chem. 2011 Jan;16(1):51-61.