The cartoon structure of the silkworm moth GOBP2 bound to an analogue of its sex pheremone.
New research announced today, Wednesday 30th September, by a team of leading scientists working with the UK’s national Synchrotron, Diamond Light Source, could have a significant impact on the development and refinement of new eco-friendly pest control methods for worldwide agriculture. Published in the Journal of Molecular Biology, the study was carried out by Dr Jing-Jiang Zhou and colleagues at the world’s oldest agricultural research centre and the largest UK facility, Rothamsted Research, in collaboration with Professor Nick Keep's group from the Institute of Structural and Molecular Biology at Birkbeck, University of London.
Dr Jing-Jiang Zhou, Senior Research Scientist in insect molecular biology at Rothamsted Research, studies insect olfaction and chemical ecology at the molecular level.
“Using Diamond Light Source’s intense X-rays, we unravelled the detailed mechanisms linked to pheromone detection which dictates mating behaviour in silkworm moths. They are a model organism and any new insights into the working of their olfactory system has repercussions on our global understanding how insects locate mates and their hosts.”
Dr Jing-Jiang Zhou, Rothamsted Research
Solving this protein structure also represents a significant achievement in the advance of structural biology in the UK and it marks the 100th new structure identified at Diamond since its opening in 2007. Professor Dave Stuart is Life Sciences Director at Diamond.
“It is a milestone and it illustrates the fascinating range of structural biology being undertaken in the UK. Congratulations to the Rothamsted and Birkbeck groups; thanks to productive groups like these, there is currently an exponential growth in the number of structures solved at Diamond.”
Prof Dave Stuart, Diamond Light Source
The importance of understanding how insects ‘smell’ and how the chemical signals are recognised is useful for many things, but especially for pest control in agriculture. Determining the composition and processes behind the olfactory functions of insects feed directly in to the development and refinement of new pathways to influence insect host locating behaviours. Plants use chemical signals to repel and attract insects and by harnessing a detailed understanding of the signals, farmers can plant companion species to create ‘odours’ that would make an area very unattractive or attractive to insects according to what they require. This is more commonly known as the push-pull system.
|Dr Jing-Jiang Zhou and Professor Nicholas Keep with a model of the structure|
Dr Jing-Jiang Zhou, adds: “So far, we know that odorant binding proteins [OBPs] within the organism pick up pheromones at pores on the outside of the antenna and carry them through a watery layer to the nerve endings. But it is not clear whether they simply transport and release molecules which bind to olfactory receptors or whether they form a specific OBP- pheromone complex which then activates the receptor. The structures we determined using the crystallography capabilities at Diamond give us a view of how these processes work.”
|Dr Jing-Jiang Zhou, Prof Gerd Materlik and Prof Dave Stuart in Diamond House|
Dr Zhou concludes: “It’s not just the farming community which stands to benefit from this work. These new insights will be fed into the development and refinement of biosensors where detection sensitivity is paramount in areas like blood tests. One of our spin-off companies are also investigating how bees can detect some small quantities of explosives and stand to benefit from any knowledge we generate.”
Rothamsted Research Institute is funded by BBSRC and Dr Zhou’s project is funded by a BBSRC SCIBS initiative grant ‘Defining the chemical space for ligands of odourant- binding proteins Ref:BB/D005892/1.
Diamond Light Source is funded by the UK Government through STFC and by the Wellcome Trust. Diamond is a platform for research for the Research Councils and in particular for AHRC, BBSRC,EPSRC, MRC and NERC.
Editor’s Note: The Artist, Lizzie Burns has created a model of this 100th protein structure to be solved at Diamond - photographs will be available from 30.09.09.
Life Sciences Director at Diamond Light Source, Professor Dave Stuart; Senior Research Scientist, Dr Jing-Jiang Zhou from the Centre for Sustainable Pest and Disease Management in the Insect Molecular Biology Group at Rothamsted Research and Professor Nicholas H. Keep, the Professor of Biomolecular Science Crystallography in the Institute for Structural and Molecular Biology at the Department of Biological Sciences Birkbeck, University of London are available for interview on request.
The work is published in Journal of Molecular Biology 2009 Jun 12;389(3):529-545. Using the structures determined at Diamond, the data disagrees with previous claims that one of the Odorant Binding Proteins expressed in the moth’s, known as BmorPBP1 bound only to bombykol and that another OBP, known as BmorGOBP2, bound to compounds from the host plants. In fact, both bind to bombykol and bombykal, but BmorGOBP2 is capable of differentiating between them and give us new insights into how this complex olfactory function in the silkworm moth actually works.
Silkworm moth or Bombyx mori are being used by biologists as a model organism in the study of the moth and butterfly family, which is known as Lepidopterans and includes over 180,000 species.
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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