Three day Workshop on Membrane Protein Production, Crystallisation and Crystallography - June 2009
Information on the course: This workshop brings together renowned scientists expert in the production, crystallisation and structure determination of membrane proteins. The workshop is being held in the unique environment of the Diamond Light Source and will include lectures, a tour and poster sessions. Participants are strongly encouraged to discuss their own research interests during the course. The course timetable is below.
Location: Diamond Light Source, Didcot, Oxfordshire, UK.
Date: 2nd to 4th June 2009.
Sponsored by: The European FP6 training program E-MeP-Lab.
Organisers: Dr Liz Carpenter (liz.carpenter@imperial.ac.uk), Dr Konstantinos Beis (kbeis@imperial.ac.uk), Dr Alexander Cameron (a.cameron@imperial.ac.uk), Membrane Protein Laboratory, Imperial College and Diamond Light Source.
- Membrane protein expression and purification
- solubilisation and stability
- High throughput methods for membrane protein expression
- Crystallisation of membrane proteins
- Rational membrane protein screen design
- High throughput crystallisation and robotics
- Lipidic cubic phase crystallisation
- Data collection with synchrotron radiation with difficult crystals
- Solving membrane protein structures
- Applications to attend the course should be sent to Mrs Mutsuko Grant, mutsuko.grant@imperial.ac.uk.
- Please send brief details of your experience to date, current research project and which areas you would particularly like to be covered during the course.
- Attendance will be limited to 30 participants.
- All applicants will be asked to provide an abstract and a poster, w hich will be displayed in the atrium in Diamond House during the course. There will be prizes for the best posters. In addition there will be time for some participants to give 15 min talks about their own research.
- Please send your applications by 20th April, 2009 at the latest.
- We will let you know by the beginning of May if you have a place on the course.
Costs: The course is funded by the E-MeP lab training grant. Registration, accommodation and subsistance will be covered by E-MEP-Lab. Travel will also be covered for non-British European Union participants. British and non-EU participants will have to pay for their own travel.
Accommodation: Accommodation will be provided in Ridgeway House, the synchrotron hostel or in local hotels.
Travel information: Information on how to travel to Diamond.
Lectures will be held in room 1.132, in the synchrotron ring.
Queries: If you have any questions please constact the organisers, Dr Liz Carpenter (liz.carpenter@imperial.ac.uk), Alex Cameron (a.cameron@imperial.ac.uk) and Kostas Beis (kbeis@imperial.ac.uk).
| 1st June, 2009 | Registration and Dinner | |
| 18.30 – 21.00 | The Atrium, Diamond House | Registration and drinks reception, followed by dinner in the on-site restaurant |
| 2nd June, 2009 | Workshop day 1 – Expression and Purification | |
| 9.10 – 9.15 | Liz Carpenter, MPL and Imperial | Introduction |
| 9.15 – 9.30 | Louise Johnson, Diamond and MPL | Welcome |
| 9.30 – 10.30 | Bernadette Byrne, Imperial College | Introduction to membrane proteins, lipids and detergents |
| 10.30 – 11.00 | Tea and coffee | |
| 11.00 - 12.00 | David Drew, Imperial College | High throughput fluorescence-based optimization of membrane protein overexpression and purification for structural studies |
| 12.00 – 1.00 | Steve Baldwin, Leeds University | High throughput expression and screening of membrane protein in E. coli |
| 1.00 – 2.00 | Lunch | Poster session and commercial stands in the Atrium |
| 2.00 – 2.30 | Kostas Beis, Imperial and MPL | Expression of outer membrane proteins |
| 2.30 – 3.30 | Chris Tate, LMB, Cambridge | Expression and stabilisation of membrane proteins |
| 3.30 - 4.00 | Tea and coffee | |
| 4.00 - 5.00 | Participants | Short presentations |
| 5.00 - 6.00 | So Iwata, Imperial College London | Membrane Protein Structural Biology |
| 6.00 - 7.00 | Posters | |
| 7.00 – 7.30 | Coaches to local restaurant | |
| 7.30 – 10.00 | Dinner in local restaurant | |
| 3rd June 2009 | Workshop day 2 Membrane Protein Crystallisation | |
| 9.00 – 9.45 | Simon Newstead | Crystallisation and designing crystallisation screens for membrane proteins |
| 9.45 – 10.30 | Liz Carpenter | Robotics and high throughput crystallisation in the MPL at Diamond |
| 10.30 – 11.00 | Coffee/Tea | |
| 11.00 – 12.00 | Bjorn Pedersen, Aarhus, Denmark | Lessons learned from solving structures of P-type ATPases |
| 12.00 – 1.00 | Christine Ziegler, Frankfurt | TBA |
| 1.00 – 1.45 | Lunch | and posters in the Atrium |
| 1.45 - 3.15 | Tour of the Diamond ring, beamlines and the Membrane Protein Laboratory | |
| 3.15 – 3.45 | Kostas Beis, Imperial | Crystallisation and selenoMet preparation of Wza |
| 4.45 – 4.15 | Participants | Short presentations |
| 4.15 - 4.45 | Tea and Coffee | |
| 4.45 - 5.45 | Gergely Katona, Gothenburg, Sweden | Structure and dynamics of photosynthetic reaction centres in lipidic sponge phase crystals |
| 6.15 – 6.45 | Coach to Oxford | |
| 6.45 – 8.00 | Stroll around Oxford | |
| 8.00 – 10.30 | Dinner in restaurant in Oxford | |
| 4th June 2009 | Workshop day 3 - Crystallisation continued... | |
| 9.00 – 10.00 | Martin Caffrey, Limerick, Ireland | Lipidic cubic phase crystallisation |
| 4th June 2009 | Workshop day 3 - Crystallography of Membrane Proteins | |
| 10.00 – 10.45 | Alex Cameron, ERATO, Imperial | Introduction to crystallography for membrane proteins |
| 10.45 - 11.00 | Coffee/Tea | |
| 11.00 – 11.45 | Gwyndaf Evans, Diamond | Data collection with synchrotron radiation with difficult crystals |
| 11.45 – 12.45 | Ricarda Hilf, Zurich, Switzerland | Case study: Pentameric ion channels |
| 12.45 – 1.30 | Lunch | |
| 1.30 – 2.00 | Alex Cameron, ERATO, Imperial | Case study: Solving Mhp1 |
| 2.00 – 3.00 | Richard Cogdell, Glasgow, Scotland | Learning how to determine the structure of membrane proteins: experiences gained from studies of purple bacterial antenna complexes |
| 3.00 – 3.15 | Quiz | |
| 3.15 – 3.30 | Tea and coffee | |
| 3.30 – 3.45 | Course assessment | |
| 3.45 – 4.00 | Poster prizes | |
| 4.00 Close of meeting | ||