Many of our expression and purification techniques are based on published work. Please see the following papers for discussion of the methods we use:

• GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. David Drew, Simon Newstead, Yo Sonoda, Hyun Kim, Gunnar von Heijne, So Iwata, Nat Protoc, 3, 784 - 798, (2008).
• High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae. Simon Newstead , Hyun Kim , Gunnar von Heijne , So Iwata , David Drew, Proc Natl Acad Sci U S A. 104, 13936-13941 (2007).
• A high-throughput method for membrane protein solubility screening: The ultracentrifugation dispersity sedimentation assay. Daniel A P Gutmann, Eiichi Mizohata, Simon Newstead, Sebastian Ferrandon, Peter J F Henderson, Hendrik W van Veen, Bernadette Byrne, Protein Sci., 16, 1422 - 1428 (2007).
• Optimization of membrane protein overexpression and purification using GFP fusions. David Drew, Mirjam Lerch, Edmund Kunji, Dirk-Jan Slotboom, Jan-Willem de Gier. Nature Method 3, 303 - 313 (2006)