Soft X-ray Full-field Imaging and Scanning Microscopy

Soft X-ray Full-field Imaging and Scanning Microscopy

Organisers:  Burkhard Kaulich and Liz Duke 
 
This workshop will cover the current status, recent achievements and future potential of beamlines B24 and I08 with the aim of identifying opportunities for further cooperation. The talks will give status updates for each of the beamlines, highlighting their capabilities and establishing their complementarity with other X-ray techniques. Alongside these presentations will be the latest scientific results including those demonstrating the added value gained by the correlation of X-ray data with information from other modalities such as light and electron microscopy.
 

Programme

Wednesday 2nd September 2015

10:30 Registration
11:00 Welcome and Facility Update
11:45 Poster Session
12:15 Lunch
12:45 Poster Session
  Workshop Session 1: Cryo Full-field X-ray Microscopy I
13:30 - 13:45 Liz Duke: A brief intriduction to Cryo Soft X-ray Microscopy
 13:45 – 14:25 

"Correlative cryo-fluorescence and cryo-soft X-ray tomography of autophagy in whole, unstained mammalian cells" Marie Charlotte-Domart, The Francis Crick Institute

14:25 – 14:55 "MISTRAL - the soft X-ray microscope at the ALBA Synchrotron Light Source" Tanja Dujic, ALBA 
15:00 – 15:30 Coffee break

 

Workshop Session 2: Scanning X-ray Microscopy I
15:30 – 16:00 "Investigating the iron will of Alzheimer’s disease" Neil Telling, Keele University
16:00 – 16:25 "Deciphering solar system history by analysis of dust from comets and asteroids" George Flynn, New York State University/ NASA
 16:25 – 16:45 "An overview of the software mapping project for Diamond mapping beamlines" Mark Basham, Diamond
17:00 Move to Lecture theatre
17:15 Poster prize & Keynote speech
18:30 Evening reception & Dinner


Thursday 3rd September 2015

  Workshop Session 3: Cryo Full-field X-ray Microscopy II
09:30 - 10:00 "Cryo-correlative light and X-ray microscopy (cryo-CLXM) workflow for the study of mammalian cells in near-native state" Raffa Carzaniga, The Francis Crick Institute
10:00 - 10:30 "An Update on Progress on the Cryo Full field soft X-ray Microscopy beamline, B24, at Diamond" Liz Duke, Diamond
10:30 - 11:00 Coffee Break
  Workshop Session 2: Scanning X-ray Microscopy II
11:00 – 11:30  "Soft X-ray ptychographic imaging of human chromosomes at the Nanosurveyor beamline at the Advanced Light Source" Joerg Schwenke, RCaH 
11:30 – 12:00 "Chemically distinct iron nanoparticle phases
in glacial meltwaters and icebergs"
Jon Hawkings, Bristol University
12:00 – 12:30 "The DLS Scanning X-ray Microscopy project" Burkhard Kaulich, DLS 
12:30  Lunch

 

Workshop Session 3: Complementary techniques in conjunction with X-ray Microscopy

13:30 – 14:00 "Egress of malaria parasites from the infected blood cell visualised by electron tomography, fluorescence microscopy and X-ray tomography" Vicky Hale, Birkbeck College University of London
14:00 – 14:30 "A cryogenic super-resolution microscope for B24" Mick Phillips, Diamond/ Oxford University
 14:30 – 15:00 "Following Abeta: Novel approaches to examine internalisation, trafficking and effects of oligomeric species in hippocampal neurons" Karen Marshall, University of Sussex
15:00- 15:30 Beamline visits
15:30  Finish