Available Equipment

Spectrophotometers/Spectrometers

Some general comments on Spectrophotometers/Spectrometers

NanoDrop® ND-1000 Spectrophotometer

This is a full-spectrum spectrophotometer (UV and visible spectrum, 220-750 nm) for measuring the absorbance of DNA, RNA, proteins and dyes this allowing for measurement of small samples volumes.

Sample measurements are possible over a wide range of concentrations: -
 
• Double-stranded (ds) DNA: 2-3700 ng/µl
• Single-stranded (ss) DNA: ≤ 2400 ng/µl
• RNA: ≤3000 ng/µl
• Protein: 0.10-100 mg/ml BSA (at 280 nm)

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Ultrasonic Scientific HR US 102 Range 2.5 - 18MHz

The spectrometer is equipped with two 1ml cells, which can be used independently or in differential measurements. Two specialised cells for analysing semi-solid materials, one cell is of volume 3ml with the second being of volume 1.5ml. Each cell can be used to analysis a raft of materials including: -

• Biological cells
• Pastes
• Waxes
• Fats
• Gels
• Suspension emulsions
• Highly attenuating samples.
• Types of analysis which can be monitored using this instrument are molecular structural changes, elasticity, thermal transitions, chemical reactions, aggregation, crystallisation etc.

Technical parameters of the instrument include:

• Frequency Range from 2.5MHz to 18MHz (cells with extended frequency range are available on request)
• High-Resolution & Reproducibility (down 10-5 % for velocity and 0.2% Attenuation)
• Vast Temperature Range -20°C to 120°C
• Absolute Temperature Control down to 0.01°C
• Variety of Measurement Regimes:
o Temperature Ramp Mode
o Kinetic Mode
o Multi-Frequency Mode

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LAMBDA 950 UV/Vis/NIR Spectrophotometer

Measurements in the 190-3300 nm range; permits the acquisition of spectra from almost any type of sample.  The current suite of sampling accessories permits: -
• Acquisition of transmission (variable angle),
• Diffuse transmission,
• Specular reflectance (variable angle: absolute and relative),
• Diffuse reflectance,
• Total reflectance spectra. 

A small spot focusing accessory permits analysis of samples as small as 2 mm x 2 mm without a significant decrease in S/N. and for applications such as: -
• Highly reflective anti-reflective coatings,
• Colour correction coatings,
• Bandpass characteristics of UV, Vis and NIR filters.

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Lambda 6505 - Range 190-1100nm

Perkin Elmer Lambada 6505

The UV/Visible range unit operates at 190-1100nm.
• Wavelength Range (nm): - 190-1100nm.
• Resolution (nm): - 0.1nm.
• Accuracy (nm): - ± 1.0nm
• Bandwidth (nm): - 1.8nm

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LAMBDA 650S UV/VIS - Range 190 - 900nm

For applications such as: -

• Hghly reflective and anti-reflective coatings.
• Colour correction coatings.
• Bandpass characteristics of UV, Vis and NIR filters.

This system is for high performance UV/Vis system, measurements between 190 nm and 900 nm. Applications include: - 

• Colour analysis of paints and textiles,
• Turbid solutions,
• Enzyme kinetics in life science,
• Biochemistry,
• Pharmaceutical laboratories.
• For ASTM, CIE, and DIN measurements.

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LABRAM HR Band - 0.3cm^-1 - 1cm^-1

Measurements at high, medium or even low spectral resolution. The high resolution mode is used for subtle band analysis such as:

• Phase (crystalline/amorphous), of proteins,
• Hydrogen and weak bonding forces and
• Semiconductor stress measurements

Band analysis in the order of 0.3 cm-1 to 1 cm-1 is particularly suited to the HR mode.
This high resolution system offers: -

• High, medium and low resolution multichannel spectral modes.
• Fluorescence and luminescence measurements.
• True confocality - maximum spatial resolution and better defined images.
• Multiple laser capability, from visible to near IR (specially extended NIR and UV versions).
• Focal lengths of 300mm
• Broad laser source
• Multiple detection options
• Near-IR detection is useful for luminescence measurements (eg. for semiconductor materials) and analysis with either 830 nm or 1064 nm excitation.

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