I have a multi-disciplinary expertise in biology (biological/medical sample preparation, cell culture, microtomy, immunohistochemistry, immuno-fluorescence, medical laboratory work from previous experience) and in IR techniques (IR spectroscopy / imaging including data collection and analysis in OPUS, Cytospec, Unscrambler softwares) which allows me to provide support to the beamline users and give advice on the best approach for sample preparation, IR data analysis and other research techniques to get the best results from their experiments.
Fourier Transform Infrared (FTIR) spectroscopy/imaging allows for qualitative and quantitative analysis of the basic components of biological tissues. This technique is quickly approaching its utility as a tool for molecular histopathology by detecting subtle chemical changes in human tissue samples. It has been used to image normal and cancer cells and has proven its potential to be implemented as a new diagnostic tool for early indications of cancer. The use of high brilliant synchrotron radiation (SR) clearly improved IR measurements on small sample structures with a signal-to-noise ratio unreachable by conventional sources.
Current research interests
- I am particularly interested in glioma brain tumor vascularisation analysis by IR spectroscopy and continuing a collaboration with University of Bordeaux on the subject. Applying FTIR micro-spectroscopy using SR source for the examination of glioblastoma vascularisation could provide detailed spectral analysis on small features like micro blood vessels in view of helping to determine any specific biomolecular markers of these angiogenic tumors.
- I am also interested in analysing fixed and living cells using the SRIR microspectroscopy to determine the best approach and optimal conditions for cells during the measurement within different environments (using microfluidic devices in static and flow mode, also temperature controlled).
- I am involved in the beamline developing projects requiring the highest diffraction limited spatial resolution in IR microscopy/imaging and the signal-to noise improvement allowed by SR on micrometric scale (e.g. Use of FPA and SR, micro-ATR techniques…).