Gianfelice Cinque

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Dr Gianfelice Cinque is Principal Beamline Scientist for the Multimode InfraRed Microscopy And Imaging (MIRIAM) beamline B22. He joined Diamond at the start of B22 project in 2006 from the INFN labs of Frascati in Rome, where he was responsible for the Soft X-ray beamline and previously on the IR beamline.
He is also currently Coordinator for the Soft Condensed Matter Village.

Email: gianfelice.cinque@diamond.ac.uk
Tel: +44 (0) 1235 778410

Key Research Area

Key Research

My main research focus is on IR microspectroscopy in (bio)physics and biomedicine, synchrotron radiation spectroscopy and imaging, and on the development of near field IR imaging and Coherent SR THz spectroscopy techniques.

Research Expertise

  1. Research Expertise
  2. Collaborators
  3. Publications
  4. Biography
Research Expertise -

Current Research Interests

InfraRed absorption spectroscopy is a non-destructive and quantitative analytical probe of the molecular composition especially of soft/condensed matter. Via an IR microscope the 2-dimensional molecular distribution is also accessible at microscopic scale for the benefit of a variety of researches, from biomedicine to new material sciences or cultural heritage. Its coupling to a broadband and bright Synchrotron Radiation source like Diamond boosts the IR microprobe molecular sensitivity beyond the capabilities of conventional methods and the spatial resolution to the diffraction limit.

My scientific activity at the MIRIAM beamline covers several collaborative researches in Synchrotron Radiation IR: BioMedicine - e.g. assessing chemotheraphy time-to-dose response at single cell level or searching for stem cells IR biomarkers for their location in tissue sections; Material Science - vibrational analysis of catalysers microcrystal or of composite/polymeric material microstructure under stress-strain; Archaeology and Cultural Heritage – elucidating the physicochemistry of ancient (XV century) painting fragments in terms of environmental changes and historical pigment evolution. State-of-the-art SR based IR microanalysis is by scanning microscopy, particularly advantageous in confocal mode. One of my current research focus is to develop full field IR microscopy via broadband SR illumination in order to provide fast IR imaging at high magnification (74x), and optimal molecular map oversampling for ultimate resolution retrieval.

THz spectroscopy can probe elusive low energy excitations, e.g. molecular collective modes or conformational changes, and superconductors gaps. My THz activity uses the intense Coherent Synchrotron Radiation emitted at Diamond in low alpha mode and MIRIAM set up allowing the broadest spectral range (<3 THz) available at SR facility.

Alongside optical methods, my major project is on near field IR spectroscopy: based on innovative detection method via submicron cantilever tips, it will open the field to sub-IR wavelength spectroscopy e.g. molecular imaging of living cells at organelle scale.

As Principal IR Beamline Scientist, I am responsible of operations and research activity at the Multimode InfraRed Microscopy And Imaging (MIRIAM) beamline B22 at Diamond.

Since November 2013 I am the coordinator of the Soft Condensed Matter Village.

 


 

Collaborators - +
  • Institute for Science & Technology in Medicine at Keele University, Cancer Centre at City General University Hospital of North Staffordshire - Stoke-on-Trent UK Synchrotron based FTIR microspectroscopy to study the effects of drugs on lung cancer cell clones co-supervisor of PhD student together with Dr Josep Sulé-Suso

  • Manchester Interdisciplinary Biocentre, Manchester University, Manchester UK IR Microspectroscopy of living cells (differentiation of Human Pluripotent Stem Cells by retinoic acid and Synthetic Retinoid Analogues) and development of combined microfluidic single cell SRIR microscopy stage for use at Diamond research collaboration with Prof Peter Gardner (until 2013 EPSRC grant for PhD shtudentship)


 

  • ChELSI Institute, University of Sheffield, Sheffield UK Department of Chemical Engineering & Biotechnology, University of Cambridge, Cambridge UK Absorption spectroscopy (protein hydration layer and molecular glass relaxation) using THz Coherent Synchrotron Radiation research collaboration with Dr Dr Robert Falconer and Dr Axel Zeitler


 

  • Dr Giuseppe Bellisola and Dr Claudio Sorio, Department of Pathology and Diagnostics, Policlinico G. Rossi and University of Verona, Verona (I), Cystic fibrosis cell pharmacological correction investigation and leukemic cell drug resistance/sensitivity recognition by Synchrotron IR microspectroscopy and unsupervised data analysis scientific collaboration, beamtime supported by EU



  • Prof Ganesh Sockalingum, Université de Reims Champagne-Ardenne, Reims (F) and Dr Josep Sulé-Suso -Keele University and University Hospital of North Staffordshire, Stoke-on-Trent, Synchrotron radiation-based InfraRed microspectroscopy analysis of extracellular interactions and cancer cell grown process in 2-D and 3-D model collagen matrix, Scientific collaboration with student exchange supported by the French Embassy in London



Dr Nativitat Salvadó Dpt. d’Enginyeria Química, Universitat Politècnica de Catalunya, Barcelona, Spain Analytical study on the ageing mechanisms of varnishes and coatings in 17th to 18th European altarpieces by SR IR microprobe with optimization of the sample preparation, Scientific collaboration, PhD student exchange supported by DLS


 

Publications - +
  • Analysis of the Hydration Water around Bovine Serum Albumin Using Terahertz Coherent Synchrotron Radiation J. W. Bye, S. Meliga,D. Ferachou, G. Cinque, J. A. Zeitler and R. J. Falconer in press on J Phys Chemistry (2014), available on line
     
  • The identification of cystic fibrosis (CF) cells and their pharmacological correction by mid-infrared microspectroscopy and unsupervised data analysis methods G. Bellisola, S. Caldrer, G. Cinque, M. Cestelli Guidi, B.M. Assaele, P. Melotti and C. Sorio in press on ScienceJet (2014), available on line
     
  • Monitoring the Activation of Copper-containing Zeotype Catalysts Prepared by Direct Synthesis using In Situ Synchrotron Infrared Microcrystal Spectroscopy and Complementary Techniques E. C. V. Eschenroeder, A. Turrina, A. L. Picone, G. Cinque, M. D. Frogley, P. A. Cox, R. F. Howe and P. A. Wright in press on Chem Materials (2013), available on line
     
  • Investigation of blood vessels in glioblastoma at a micrometric scale: a comparative study by synchrotron and conventional micro-FTIR K. Wehbe, A. Travo, S. Eimer, G. Cinque, E. Barron, G. Deleris, I. Forfar Analytical Methods (2013)
  • 5: 6925-6932 Deformation and failure mechanisms in graphene oxide paper using in situ nanomechanical tensile testing C. Wang, M. D. Frogley, G. Cinque, LQ Liu, A. H. Barber Carbon (2013) 63, 471–477
     
  • Rapid recognition of drug-resistance/sensitivity in leukemic cells by Fourier transform infrared microspectroscopy and unsupervised hierarchical cluster analysis G. Bellisola, G. Cinque, M. Vezzalini, E. Moratti, G. Silvestri, S. Redaelli, C. Gambacorti Passerini, K. Wehbe and C. Sorio Analyst (2013) 138, 3934-3945
     
  • Shades of green in 15th century paintings: combined microanalysis of the materials using Synchrotron radiation XRD, FTIR and XRF N. Salvado, S. Buti, M. Cotte, G. Cinque and T. Pradell Applied Physics A (2013), 111: 47-57
     
  • The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy G. Clemens, K.R. Flower, A.P. Henderson, A.Whiting, S.A. Przyborski, M.Jimenez-Hernandez, F. Ball, P.Bassan, G.Cinque and P.Gardner Mol. BioSyst. (2013) 9: 677-692
     
  • The effect of optical substrates on micro-FTIR analysis of single mammalian cells K. Wehbe, J. Filik, M.D. Frogley and G. Cinque Anal. Bioanal. Chem. (2013), 405:1311–1324
     
  • Isolating stem cells in the inter-follicular epidermis employing synchrotron radiation-based Fourier-transform infrared microspectroscopy and focal plane array imaging I. I. Patel, W. J. Harrison, J. G. Kerns, J. Filik, K.Wehbe, P. L. Carmichael, A.D. Scott M.P. Philpott, M.D. Frogley, G. Cinque and F. L. Martin Anal. Bioanal. Chem. (2012), 404:1745–1758
     
  • The Broadband and High Sensitivity X-Ray Beam Position Monitor of the IR beamline MIRIAM at Diamond G. Cinque, G. Howell, T. Cobb and N. Tartoni J. Phys.: Conf. Ser. (2013), 425:042007
     
  • Morphological analysis of vibrational hyperspectral imaging data J. Filik, A.V. Rutter, J. Sulé-Suso and G. Cinque Analyst (2012), 137: 5723-5729
     
  • Electric field standing wave artefacts in FTIR micro-spectroscopy of biological materials J. Filik, M.D. Frogley, J.K. Pijanka, K. Wehbe and G. Cinque Analyst (2012), 137:853-861
     
  • Optical standing-wave artifacts in reflection-absorption FTIR microspectroscopy of biological materials J Filik, M D Frogley, J Pijanka, K Wehbe and G Cinque J. Phys.: Conf. Ser. (2012), 359:012006
     
  • Recording IR spectra for individual electrospun fibers using an in situ AFM-synchrotron technique U. Stachewicz, F. Hang, R.J. Bailey, H.S. Gupta, M. Frogley, G. Cinque, A.H. Barber MRS Proceedings (2012), 1424
Biography - +

Gianfelice Cinque is Principal Beamline Scientist for the Multimode InfraRed Microscopy And Imaging (MIRIAM) beamline at Diamond since the start of B22 project in 2006. He joined Diamond from the INFN labs of Frascati (I) where he was responsible of the Soft X-ray beamline, and previously scientist at the SINBAD infrared beamline at DAFNE storage ring since 2001.

His expertise in synchrotron radiation and absorption spectroscopy is related to IR and soft X-rays experimental methods respectively for FTIR vibrational analysis in the mid-IR to THz and XANES in the energy region 1 to 4 keV.

As background, he graduated in Physics at Padua University in 1990 and had his PhD in General Physics from Ferrara University in 1994, followed by a Post Doc in Physics at Verona University. He was teaching Medical Physics at the Faculty of Medicine in Verona University from 1994 to 1997.