I04 is one of five macromolecular crystallography beamlines at Diamond. The typical working wavelength is 0.9795 Å (12.658 keV) but it is tuneable over the wavelength range 0.69 - 2.066 Å.
The beamsize can be focussed from 10 x 5 to 110 x 100 microns across the available energy range.
All MX beamlines use SPINE standard pins and Unipucks from cryocooled samples. The beamline can be accessed remotely.
We will be barcoding pucks from April 2017 onwards.
Data reprocessing now available in ISPyB. See here
New monochromator for I04. Big improvements, more to learn and deliver to users over coming months.
New remote access connection available
New BART robot installed on I04 - 37 pucks, 592 samples, 2.5x faster exchange times!
New focus options on I04. Variable beam size from 5 - 100 microns between 6 - 14 keV.
Go to GDA - Messages- Beamline notes to read the message of the day. Click here for more.
New web interface for ISPyB launched. For more information click here.
Deadline for Call for Proposals for AP16 is 25th March 2014. See here.
Loan pucks available upon request, check here
Test your network connection to Diamond with our SpeedTest
Crystal washer now available
I04 reopens to users for run 3. Now with Mini-Kappa installed in development phase.
Updated excel spreadsheet for shipments in ISPyB. Download and use the latest version.
I04 reopens to users on 25th April
I04 installed a Pilatus 6M-F detector in April 2013.
Remote access available on all MX beamlines at all times since January 2013
|Variable Focus Beam||✓|
|Fluorescence data collection||✓|
|Interleaved energy data collection||✓|
|Inverse beam data collection||✓|
|IMISX Capable for LCP in situ studies - discuss with staff before arrival ||✓|
|In situ goniometry for crystallisation plate ||✗|
|Automated strategy calculation||✓|
|Automated data processing||✓|
|Automated difference map calculation||✓|
|Automated molecular replacement||✓|
|Standard energy (Å / keV)||0.98 / 12.66|
|Flux at 12.66 keV (ph/s)||2.5 x 1011|
|Wavelength range (Å)||0.69 - 2.07|
|Energy range (keV)||6.0 - 18.0|
|Beam size at sample (µm)||10 x 5 --> 110 x 100|
|Time to collect 180o data set (minutes)||1.2 +|
|No. of pins in dewar||592|
|Sample exchange time (seconds)||18|
|Guide to samples screened/hour (user centering)||30|
+ 0.1o per 0.04 second exposure 1800 frames (shutterless)
A new compound refractive lens focussing system has been implemented on the beamline since January 2015. This provides a variable focus beam size across the available energy range. The chart below shows fluxes for beam sizes at selected energies for the current beamline configuration:
From January 2013 until December 2014 repolished mirrors delivered a focussed beam of 90 x 40 µm across all available energies. When coupled with beam shaping apertures the photon fluxes obtained at the sample during this period are shown below:
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