I02 is one of five macromolecular crystallography (MX) beamlines at Diamond. I02 is a tuneable beamline with a working wavelength range of 0.68 - 2.48 Å. The standard working energy is 12.658 keV which provides a wavelength of 0.979 Å and a focussed beam size of 80 x 20 um (FWHM). As with all the MX beamlines, I02 uses SPINE standard pins and Unipucks.
Since April 2014 I02 has changed. The area that used to be the control cabin and sample preparation space are now part of the new VMXi beamline being built. The I02 control cabin and sample prep area are now located in portacabins.
To find the beamline, please enter the synchrotron at zone 5 and walk counter clockwise (towards zone 4). The portacabins on your left, behind the spare girder, are the new I02 control cabin and sample preparation lab.
New remote access connection available
Go to GDA - Messages- Beamline notes to read the message of the day. Click here for more.
New web interface for ISPyB launched. For more information click here.
Deadline for Call for Proposals for AP16 is 25th March 2014. See here.
Loan pucks available upon request, check here
Test your network connection to Diamond with our SpeedTest
461 structures have been published from I02: 34 so far in 2013.
Updated excel spreadsheet for shipments in ISPyB. Download and use the latest version.
I02 will take first users after the shut down on Friday 21st April.
Remote access available on all MX beamlines at all times since January 2013
I02 has a new control cabin and a new sample preparation cabin.
|Detector type||Pilatus 6M|
|Standard working wavelength (Å / keV)||0.979 / 12.658|
|Maximum resolution (Å) at this wavelength||1.09|
|Available wavelengths (Å)||0.62 - 2.48 (0.5 to 0.69 upon request)|
|Available wavelengths (keV)||5.0 - 20.0 (up to 25 upon request)|
|Maximum attainable resolution (Å)||0.8|
3.7 x 1012 ph/s (200 micron aperture)
4.4 x 1011 ph/s (20 micron aperture)
1.7 x 1011 ph/s (10 micron aperture)
|Flux Details||Measured @ 12658 eV and 300 mA ring current|
|Absorption edge scans||Optimised SAD and MAD experiments|
|Focussed Beam Size (µm)||82 x 19 (FWHM)|
|Slit Size (µm)||200x200 to 25x25|
|Sample changer||Rigaku ACTOR with unipucks - exchange time < 40 sec|
|Pins and Pucks||SPINE standard pins and Unipucks|
Humidity Controller for room temperature data collection (HC1)
|Available on request - now integrated into endstation setup|
Please discuss your requirements with a member of the beamline team before your experiment.
Click here for a poster showing how to interpret the beam size display on I02.
Click here for a poster presenting the beam properties in July 2014.
Every day a member of the beamline team will set up the beamline for use. Information from this set up can be found in your visit directory (yyyymmdd_setupDetails.txt). In addition a printed copy of this information is filed on the beamline and archived.
At the start of each run a more comprehensive check of the beamline is made. This information is displayed by poster at the beamline.
Beam Focus Modes
Two beam focus modes are currently available. The figure above shows the beam on the scintillator for each mode. The figure below shows the beam profiles in the horizontal and vertical for each focus mode. The table below shows the analysis of the beam profiles.
The I02 beam can be attenuated. The plot below left shows the flux achieved plotted agains the transmission requested. This indicates the degree of precision in the attenautors. The flux is dependent on energy and a plot of the flux across the energy range will be posted here soon.
Using a Pilatus detector means data can be collected quickly. This can be achieved by adjusting the exposure time down and increasing the transmission to provide the same dose per degree. For example the following three data collections would have the same radiation dose:
To confirm higher rotation speeds to not detract from data quality 3 glucose isomerase crystals were collected in three different ways. In the table below the average Rmerge and I/Sigma values are given with the standard deviation given in parentheses. This indicates that the data are of comparable quality.
Dectris recommend collecting finely sliced data. Omega oscillation should not exceed half the XDS mosaicity (reflecting range).
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