Macromolecular Crystallography (MX)

The Diamond MX village currently comprises five state-of-the-art undulator beamlines - three high brilliance MAD beamlines (I02, I03, I04), a fixed wavelength beamline (I04-1) and a microfocus MAD beamline (I24). We offer a wide range of facilities and capabilities including microbeams, in situ (in-plate) screening and data collection, sample humidity control, UV-Vis spectroscopy and data collection from pathogenic samples.

 

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Microfocus

Specialised high flux microfocus beams for microcrystal data collection or the illumination of subvolumes of larger inhomogeneous crystals.

I24
Remote Access

The highly automated beamlines enable data collection from anywhere in the world via remote access. Systems are in place for shipping samples to and from Diamond Light Source. New users can borrow pucks and tools.

In situ Data Collection

In situ data collection and sample characterization directly from crystals in their crystallization plates.

Sample Humidity Control

Control of the sample crystal's relative humidity with the aim of improving diffraction quality through improved packing of protein molecules constituting the lattice.

Spectroscopy

On-line and off-line UV-Vis spectroscopy of macromolecular samples to study enzyme reaction mechanisms and radiation damage.

Mini Kappa

Crystal reorientation to optimize data collection strategies for anomalous diffraction data collection and low symmetry spacegroups, or very long axis unit cells.

Biocontainment

All beamlines are designated bio containment level 1. For containment level 2 samples beamlines I03 and I24 can be used and beamline I03 can be configured to run at biocontainment level 3.

I02 MX

High throughput and highly automated beamline for optimised MAD and SAD experiments.

Beamsize: 90 x 20

Wavelength: 0.5Å - 2.5Å

Detector: Pilatus 6M-F

I03 MX

High throughput and highly automated beamline for optimised MAD and SAD experiments. Capable of accepting CL3 type experiments on crystals of pathogens.

Beamsize: variable

Wavelength: 0.5Å - 2.5Å

Detector: Pilatus 6M-F

I04 MX

High throughput and highly automated beamline for optimised MAD and SAD experiments. 

Beamsize: 85 x 40

Wavelength: 0.59Å - 2.48Å

Detector: Pilatus 6M-F

I04-1 Fixed wavelength MX

High throughput and highly automated fixed wavelength SAD beamline for macromolecular crystallography.

Beamsize: 60 x 50

Wavelength: 0.92Å (fixed)

Detector: Pilatus 2M

I24 Microfocus MX

High throughput variable microfocus beamline for optimised MAD and SAD experiments on crystals down a few microns in size.

Beamsize: 5x5 - 50x40 (variable aspect ratio)

Wavelength: 0.62Å - 1.77Å

Detector: Pilatus 6M-F

 

Beamline
I02 I03 I04 I04-1 I23 I24
Wavelength range (Å)
 0.5 - 2.5  0.59 - 2.3 0.62 - 2.48 0.92 (fixed)   0.62 - 1.77
Energy range (keV)
5.0 - 25.0 5.2 - 21.0 5.0- 20.0 13.5 (fixed)   7.0 - 20.0
Default settings (Å/keV)  0.98 / 12.66 0.98 / 12.7 0.98 / 12.66 0.92 / 13.53   0.97 / 12.8
Flux (ph/s) in full beam at default energy at 300 mA 4.0 x 1012 2.5 x 1012 3.2 x 1012 .5 x 1011   1.4 x 1012
Default beamsize (µm) 80 x 20 80 x 20 95 x 40 60 x 50   8 x 8
Full beam size at sample (µm)

80 x 15 / 85 x 44 / 48 x 18

 

80 x 20 90 x 30 100 x100 55 x 10   60 x 50  

8 x 8 --> 50 x 40

5 x 5 (with 1011 ph/s)

Available apertures (µm) 10,20 and 200

20, 50, 100 (cryo)

10, 30, 100 (in-situ)

20, 50, and 200 10, 20, 30, 50 and 70    
Detectors (Pilatus type)

P6M-F

(25 Hz)

P6M-F

(25 Hz)

 P6M-F

(25 Hz)

P2M

(30Hz)

Custom P12M

 

P3-6M

(100 Hz)

Number of unipucks / SPINE pins  10 / 160 10 / 160 10 / 160 9 / 144   9 / 144
Maximum samples / hour 30 30 30 30   30
Typical samples / hour 15 - 20 15 - 20  15 - 20 15 - 20   15 - 20 

News in Pictures

  • Loan pucks available upon request.
  • New web interface to ISPyB has been launched - please take a look before your visit!
  • New 'Message of the Day' functionality launched in GDA.
  • I04-1 has made undulator changes to increase photon flux.
  • Science Highlight: Scientists unlock the structure of elusive 'stress' protein.
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Beamline Manuals