Michele Darrow

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Michele Darrow is a Post Doctoral Research Associate working on beamline B24.  Michele joined Diamond in September 2015 after attending Baylor College of Medicine.

Email: michele.darrow@diamond.ac.uk
Tel: +44 (0) 1235 7787525

Key Research Area

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Research summary

  1. Research
  2. Collaborators
  3. Publications
Research -

Electron Cryotomography, X-ray Cryotomography, Protein Misfolding Disorders.

X-ray cryotomography is an exciting technique that allows for the imaging of whole cells without dehydration, fixation or staining. This provides the cellular context necessary to answer complex biological questions.

My projects generally focus on visualizing the internal workings of healthy and pathological cells to identify differences between the two. More specifically, I will be looking at viral infection of cells, and also the effects of misfolded proteins.
 

Currently, most of my projects are in collaboration with other labs. So the samples are prepared elsewhere and shipped to me for light and x-ray imaging. In addition to data collection, I will also process the collected data.

 

Collaborators - +

 

In collaboration with researchers at Baylor College of Medicine, I am imaging cells infected with either HSV-1 or rotavirus, to answer specific questions regarding interactions between the virus particles and various cellular components. Additionally, I will image cells expressing the toxic misfolded protein in Huntington's Disease and Alzheimer's Disease, in order to better understand where these aggregates form and what cellular components they interact with.

Publications - +

Darrow, MC, OA Sergeeva, JM Isas, J Galaz-Montoya, M Schmid, JA King, R Langen, & W Chiu. (2015) Structural Mechanisms of Mutant Huntingtin Aggregation Suppression by Synthetic Chaperonin-like CCT5 Complex Explained by Cryo-electron Tomography. J. Biol. Chem. 290(28): 17451-61.

Darrow, MC, Y Zhang, BP Cinquin, EA Smith, R Boudreau, RH Rochat, MF Schmid, Y Xia, CA Larabell & W Chiu. (In Review) Visualizing red blood cell sickling and the effects of inhibition of SphK1 using soft x-ray cryotomography.